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  • 快速基因优发国际手机版
    通用生物为您提供5天快速基因优发国际手机版服务,节省您等待的时间,提高您的实验效率!您还在等什么?


    服务订购

    基因长度

    交货周期

    价格(元/bp)

    备  注

    <300 bp

    5个工作日

    600


    300~1200 bp

    5个工作日

    1.5


    1201~2000 bp

    7个工作日

    1.6



    ◆ 如果客户不指定载体,公司默认把客户目的基因序列克隆到PUC57上,不收取亚克隆费用。

    服务说明
    快速基因优发国际手机版需满足以下条件:
  • 非复杂序列
  • 长度≤ 2.0kb
  • 克隆至pUC57载体
  • 无需点突变及质粒制备
  • 服务订购
    我公司为客户提供全部技术资料和产品,包括:
    -COA文件
    -质粒结构图
    -约5μg冻干质粒DNA
    -测序图谱
    -含有重组质粒的甘油菌或穿刺菌一管
    特别声明:本公司承诺对客户的基因序列不做任何形式的扩散、开放或利用,对相关信息实行完全保密,公司交付产品一个月后,彻底销毁所有相关的引物、质粒和菌种。

    Peer-reviewed Citations:
    Combined SNPs and miRNAs technologies for beef traceability
    http://onlinelibrary.wiley.com/doi/10.1111/jfs.12360/full
    Q Wu, G Zhou, S Yang, X Xu, C Li - Journal of Food Safety ... Premix Ex Taq Kits (Takara Biotechnology Co. Ltd., Dalian, China). Forward primers used for RT-PCR were synthesized by General Biosystems (General Biosystems Co. Ktd., Anhui, China). The forward primer sequences of the...

    Assessment of the fusion tags on increasing soluble production of the active TEV protease variant and other target proteins in E. coli
    https://link.springer.com/article/10.1007/s12010-016-2360-9
    Xuelian Yu, Jiaqi Sun, Weiyu Wang, Li Jiang, Beijiu Cheng, Jun Fan-Applied Biochemistry and Biotechnology ...Primary and secondary antibodies as well as reagents for Western blotting were provided by Sangon Biotech (Shanghai, China). Primer synthesis and DNA sequencing were conducted by General Biosystems (Chuzhou, China)...

    Engineering thermostable (R)-selective amine transaminase from Aspergillus terreus through in silico design employing B-factor and folding free energy calculations
    http://www.sciencedirect.com/science/article/pii/S0006291X1632191X
    J Huang, DF Xie, Y Feng - Biochemical and Biophysical Research Communications ...The AT-ATA cDNA sequence containing the NcoI and XhoI restriction sites was synthesized at General Biosystems (AnHui) Co., Ltd (Chuzhou, China), and the pET28a(+) expression vector(pET28a(+)-AT-ATA) was constructed as a template for site-directed mutagenesis...

    A Vector Based on the Chicken Hypersensitive Site 4 Insulator Element Replicates Episomally in Mammalian Cells
    http://www.ingentaconnect.com/content/ben/cgt/2016/00000016/00000006/art00006
    Zhang,Xi;Zhang,Xi;Wang,Xiao-Yin;Wang,Xiao-Yin;Jia,Yan-Long;Jia,Yan-Long;Guo,Xiao;Guo, Xiao;Wang,Yan-Fang;Wang,Yan-Fang;Wang,Tian-Yun;Wang,Tian-Yun-Current Gene Therapy ...trans- gene expression. MATERIALS AND METHODS Vector Construction The cHS4 DNA sequence used in this study was artificially synthesized by General Biosystems, Inc. (Chuzhou, China) [23]. The synthesized sequences...

    TTF1, in the Form of Nanoparticles, Inhibits Angiogenesis, Cell Migration and Cell Invasion In Vitro and In Vivo in Human Hepatoma through STAT3 Regulation
    http://www.mdpi.com/1420-3049/21/11/1507/htm
    Bin Xiao, Dongjing Lin, Xuan Zhang, Meilan Zhang and Xuewu Zhang-Molecules …pcDNA3.1 plasmid of STAT3 was synthesized by General Biosystems (An Hui, General Biosystems, Inc., Chuzhou, China)...

    Matrix attachment region combinations increase transgene expression in transfected Chinese hamster ovary cells
    http://www.nature.com/articles/srep42805
    Chun-Peng Zhao, Xiao Guo, Si-Jia Chen, Chang-Zheng Li, Yun Yang, Jun-He Zhang, Shao-Nan Chen, Yan-Long Jia & Tian-Yun Wang-Sci Rep. …a 2073-bp fragment (position 94–2166) was synthesized by General Biosystems (Chuzhou, China)…

    Genome-wide analysis of Dof family genes and their expression during bud dormancy in peach (Prunus persica)
    http://www.sciencedirect.com/science/article/pii/S0304423816305738
    M Chen, X Liu, L Huan, M Sun, L Liu, X Chen, D Gao, L Li-Scientia Horticulturae …All of the primerswere designed using the Beacon Designer 7 software and synthesized by GeneralBiosystems (Anhui, China)…

    Improving thermostability of (R)-selective amine transaminase from Aspergillus terreus through introduction of disulfide bonds
    http://onlinelibrary.wiley.com/doi/10.1002/bab.1572/full
    DF Xie, H Fang, JQ Mei, JY Gong, HP Wang, XY Shen, J Huang, LH Mei-Biotechnology and Applied Science … Materials The AT-ATA cDNA from A. terreus sequence, including the NcoI and XhoI restriction sites, was synthesised at General Biosystems (AnHui) Co., Ltd (Chuzhou, China), and plasmid pET28a(+) was used for gene cloning and DNA sequencing...

    Tobacco etch virus protease mediating cleavage of the cellulose-binding module tagged colored proteins immobilized on the regenerated amorphous cellulose
    https://link.springer.com/article/10.1007/s00449-017-1772-4
    Xuelian YuJiaqi SunWeiyu WangLi JiangRuyue WangWenjun XiaoBeijiu ChengJun Fan-Bioprocess and Biosystems Engineering ... Materials and methods. Materials. Reagents used in plasmid construction and protein expression were bought from Takara (Dalian, China). Gene and primer syntheses, DNA sequencing are conducted by General Biosystems (Chuzhou, China)...
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